Neutrophil Rolling, Arrest, and Transmigration Across Activated, Surface- Adherent Platelets Via Sequential Action of P-Selectin and the &-Integrin CDllb/CDl8
نویسندگان
چکیده
Platelets bound to thrombogenic surfaces have been shown to support activation-dependent firm adhesion of neutrophils in flow following selectin-mediated tethering and rolling. The specific receptor(s) responsible for mediating adhesion-strengthening interactions between neutrophils and platelets has not previously been identified. Furthermore, the ability of adherent platelets to support the migration of bound neutrophils has not been tested. We studied neutrophil interactions with activated, surface-adherent platelets as a model for leukocyte binding in vascular shear flow and emigration at thrombogenic sites. Our results demonstrate that the &-integrin Mac-1 (CDI IblCD18) is required for both firm attachment to and transmigration of neutrophils across surface-adherent platelets. In flow assays, neutrophils from patients with leukocyte adhesion deficiency-1 (LAD-I), which lack &-integrin receptors, formed P-selectin-mediated rolling interactions, but were unable to develop firm adhesion
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